MUSCLE GROWTHResearch OnlyGHRH ANALOGGROWTH HORMONEMUSCLE

CJC-1295

Also known as: CJC-1295 DAC · Modified GRF(1-29) · Drug Affinity Complex GHRH

24.9k views/week 389 citations 0 edits Updated 4/6/2026

CJC-1295 is a synthetic analog of growth hormone-releasing hormone (GHRH) with a Drug Affinity Complex (DAC) modification that enables albumin binding in the bloodstream, extending its half-life from minutes to 6–8 days. It acts at the pituitary to increase GH pulse frequency and baseline IGF-1 levels.

STRUCTURE

Molecular Composition

FORMULA
C₁₅₂H₂₅₂N₄₄O₄₂
MOL. WEIGHT
3367.9 Da
SEQUENCE LENGTH
30 amino acids
CAS NUMBER
863288-34-0
HALF-LIFE (DAC)
6–8 days
SOLUBILITY
Water-soluble
AMINO ACID CHAIN VISUALIZATION
Y
Tyrosine
N-terminal start
NH-CO
D
Aspartate
GHRH receptor binding
NH-CO
A
Alanine
helical stability
NH-CO
I
Isoleucine
hydrophobic core
NH-CO
F
Phenylalanine
receptor contact
NH-CO
T
Threonine
chain flexibility
NH-CO
K
Lysine*
DAC conjugation site
SEQUENCEY-D-A-I-F-T-K
MECHANISMS

How It Works

🧬
GHRH Receptor Agonism
Binds and activates GHRH receptors on pituitary somatotrophs with high affinity and selectivity, triggering a surge in growth hormone secretion that mirrors the natural GH pulse pattern.
⏱️
Extended Half-Life (DAC)
The Drug Affinity Complex (DAC) covalently bonds CJC-1295 to endogenous albumin in vivo, extending its half-life from ~30 minutes (Mod GRF 1-29) to 6–8 days. This enables once-weekly dosing with sustained GH elevation.
📈
IGF-1 Upregulation
Elevated GH from pituitary stimulation drives hepatic IGF-1 synthesis. IGF-1 mediates the anabolic effects of GH — muscle protein synthesis, lipolysis, and collagen production — and is the primary downstream effector measured in research.
💡
Pulsatile GH Preservation
Unlike exogenous HGH injections which create supraphysiological flat-line levels, CJC-1295 amplifies natural GH pulses without suppressing the pulsatile pattern critical for long-term receptor sensitivity and somatotroph health.
OVERVIEW

Research Overview

CJC-1295 was developed by ConjuChem Biotechnologies as a long-acting GHRH analog for clinical investigation in GH deficiency. The key innovation is the DAC technology — a maleimide group attached to lysine at position 8 that reacts spontaneously with the free thiol groups of circulating albumin, creating a stable covalent bond that protects the peptide from enzymatic degradation and renal clearance.

While the DAC version offers extended dosing convenience (once or twice weekly), a non-DAC version (Modified GRF 1-29, or "Mod GRF") is also widely used, offering a shorter 30-minute window of GH elevation that more closely mirrors physiological GH pulses. The choice between DAC and non-DAC versions depends on the desired GH secretion pattern.

Mechanism of Action

// GHRH RECEPTOR AGONISM

CJC-1295 binds to and activates GHRH receptors (GHRHR) on anterior pituitary somatotrophs with higher affinity than endogenous GHRH. Receptor activation triggers cAMP-dependent protein kinase A signaling, increasing both GH synthesis and secretory pulse amplitude.

// IGF-1 AXIS UPREGULATION

Sustained GHRHR stimulation by CJC-1295 DAC raises baseline IGF-1 (Insulin-like Growth Factor 1) by 200–400% in clinical studies. IGF-1, produced primarily in the liver, mediates the anabolic effects of GH — muscle protein synthesis, lipolysis, and bone density maintenance.

SEQUENCE

Amino Acid Sequence

Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-NH2
DOSAGE

Dosage & Administration

INJECTABLE (SUBCUTANEOUS) — WITH DAC
DOSE
1–2 mg
FREQUENCY
Once weekly or every 2 weeks
NOTES
CJC-1295 with DAC (Drug Affinity Complex) provides a sustained half-life of 6–8 days via albumin binding. Once-weekly injection maintains stable GH pulse elevation. Most convenient protocol.
INJECTABLE (SUBCUTANEOUS) — WITHOUT DAC (MOD GRF 1-29)
DOSE
100–200 µg
FREQUENCY
3×/day (before meals and at bedtime) or combined with GHRP
NOTES
Without DAC, half-life is ~30 min. Mimics natural GH pulsatility. Best combined with a GHRP (e.g., Ipamorelin) for synergistic GH release. Inject on an empty stomach.
INJECTABLE (SUBCUTANEOUS) — STACKED WITH IPAMORELIN
DOSE
100 µg CJC-1295 (no DAC) + 200 µg Ipamorelin
FREQUENCY
2–3×/day
NOTES
The most studied combination protocol. Synergistic GH release — CJC-1295 amplifies GH pulse height while Ipamorelin triggers release. Administer 30 min before food or training.

CJC-1295 stimulates pituitary GH release rather than providing exogenous GH, preserving the natural pulsatile release pattern. This is a key safety advantage over synthetic HGH. IGF-1 levels should be monitored during longer cycles. Water retention and mild fatigue are common initial effects that typically resolve within 2–3 weeks.

CYCLING

Cycle Duration Guide

ON CYCLE
8–12 weeks
OFF CYCLE
4–6 weeks minimum

Longer cycles (up to 6 months) are used in research contexts with regular IGF-1 monitoring. The off-cycle allows pituitary sensitivity to reset and prevents downregulation of endogenous GHRH receptors. IGF-1 levels typically return to baseline within 4 weeks of cessation.

Elevated IGF-1 beyond normal physiological ranges over extended periods is theoretically associated with increased cellular proliferation risk. Monitor IGF-1 levels and consult a physician for cycles longer than 12 weeks.

Quick Reference
FORMULAC₁₅₂H₂₅₂N₄₄O₄₂
MOL. WEIGHT3,367.97 Da
LENGTH29 amino acids
ORIGINSynthetic GHRH(1-29) analog with covalent albumin-binding Drug Affinity Complex
HALF-LIFE6–8 days with DAC modification; ~30 minutes without DAC
SOLUBILITYSoluble in bacteriostatic water
CAS NO.863288-34-0
STATUSResearch Only
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TAGS
GHRH analoggrowth hormonemuscleanti-agingfat losssleep