COGNITIVEFDA ApprovedCOGNITIVE ENHANCEMENTNEUROPROTECTIONBDNF

Semax

Also known as: ACTH(4-10)-Pro-Gly-Pro · Met-Glu-His-Phe-Pro-Gly-Pro

22.1k views/week 356 citations 0 edits Updated 4/6/2026

Semax is a synthetic heptapeptide analog of the adrenocorticotropic hormone (ACTH) 4–10 fragment, developed at the Institute of Molecular Genetics of the Russian Academy of Sciences. It is approved in Russia for treating stroke, brain trauma, optic nerve disease, and cognitive disorders, with a strong BDNF-upregulating profile.

STRUCTURE

Molecular Composition

FORMULA
C₃₇H₅₁N₉O₁₀S
MOL. WEIGHT
813.93 Da
SEQUENCE LENGTH
7 amino acids
CAS NUMBER
80714-61-0
ORIGIN
ACTH(4-7) analogue
SOLUBILITY
Water-soluble
AMINO ACID CHAIN VISUALIZATION
M
Methionine
N-terminal start
NH-CO
E
Glutamate
charge balance
NH-CO
H
Histidine
receptor interaction
NH-CO
F
Phenylalanine
core pharmacophore
NH-CO
P
Proline
structural rigidity
NH-CO
G
Glycine
flexible linker
NH-CO
P
Proline
C-terminal cap
SEQUENCEM-E-H-F-P-G-P
MECHANISMS

How It Works

🧠
BDNF Upregulation
Increases brain-derived neurotrophic factor (BDNF) in the hippocampus and cortex. BDNF is the primary driver of neuroplasticity, long-term potentiation, and the formation of new synaptic connections underlying learning and memory.
Dopamine & Serotonin Modulation
Enhances dopaminergic and serotonergic transmission in the prefrontal cortex and limbic system. This drives the observed improvements in attention, working memory, and motivation.
🛡
Neuroprotection
Reduces oxidative stress and neuroinflammation following ischemic events. Clinically validated in Russia for post-stroke cognitive rehabilitation — one of the few peptides with approved therapeutic use for neurological indications.
🔬
Melanocortin Pathway
As an ACTH(4-7) analogue, Semax activates melanocortin receptors (MC2R-MC5R) in the CNS. This modulates stress response, attention allocation, and adaptive behavior — mechanisms distinct from conventional nootropics.
OVERVIEW

Research Overview

Semax was developed in Russia in the 1980s and approved by the Russian Ministry of Health in 1995. It remains one of the few research peptides with a formal approval history, having been granted status for treatment of ischemic stroke, TIA, optic atrophy, and cognitive disorders in Russia and Ukraine.

Unlike ACTH itself, Semax lacks hormonal activity (no adrenal stimulation) because its sequence does not include the corticotropic activity domain. Instead, the Pro-Gly-Pro tripeptide addition from prolyl-endopeptidase cleavage gives Semax its neuroprotective properties and significantly extends its metabolic stability compared to ACTH(4-10). It is most commonly administered intranasally, bypassing the blood-brain barrier effectively.

Mechanism of Action

// BDNF & NEUROTROPHIN UPREGULATION

Semax produces a rapid and sustained 4–8 fold increase in BDNF (Brain-Derived Neurotrophic Factor) mRNA expression in the hippocampus and cortex. BDNF is the primary driver of neuronal survival, synaptogenesis, and long-term potentiation — the molecular substrate of learning and memory formation.

// DOPAMINE & SEROTONIN MODULATION

Semax increases dopaminergic and serotonergic tone in the prefrontal cortex and limbic system, contributing to its anxiolytic and pro-cognitive effects. It upregulates DAT (dopamine transporter) expression and modulates 5-HT₂ receptor activity.

// NEUROPROTECTION VIA HIF-1α

Under ischemic conditions, Semax activates HIF-1α (hypoxia-inducible factor), triggering a neuroprotective gene expression cascade that reduces apoptosis, suppresses neuroinflammation (via NF-κB), and promotes angiogenesis in penumbral brain tissue.

SEQUENCE

Amino Acid Sequence

Met-Glu-His-Phe-Pro-Gly-Pro
DOSAGE

Dosage & Administration

INTRANASAL
DOSE
200–600 µg (0.1% solution: 1–3 drops per nostril)
FREQUENCY
1–2×/day, in the morning and/or early afternoon
NOTES
Most common route. Rapidly absorbed via nasal mucosa with direct CNS access via olfactory pathway. Avoid evening dosing to prevent sleep disruption.
INJECTABLE (SUBCUTANEOUS)
DOSE
50–300 µg
FREQUENCY
Once daily
NOTES
More bioavailable than intranasal. Used in clinical research settings. Onset is slightly slower than intranasal but systemic exposure is higher and more consistent.

Semax is a synthetic heptapeptide derived from the N-terminal fragment of ACTH (residues 4–7), extended with Pro-Gly-Pro to resist enzymatic degradation. It has been approved in Russia for stroke treatment and cognitive enhancement since the 1990s. No significant side effects noted at standard doses; stimulatory effects may cause anxiety at high doses in sensitive individuals. Available as 0.1% and 1% nasal solutions.

CYCLING

Cycle Duration Guide

ON CYCLE
2–4 weeks
OFF CYCLE
2–4 weeks

Short cycles are standard for Semax to prevent receptor adaptation and maintain consistent cognitive effects. Some researchers use it acutely (7–14 days) for specific tasks or recovery windows rather than continuous supplementation. Stacking with Selank (anxiolytic peptide) is common to balance Semax's stimulatory profile.

Quick Reference
FORMULAC₃₇H₅₁N₉O₁₀S
MOL. WEIGHT813.93 Da
LENGTH7 amino acids
ORIGINSynthetic analog of ACTH(4-10) with C-terminal Pro-Gly-Pro extension; developed in Russia
HALF-LIFE~20 minutes plasma half-life; intranasal administration preferred for CNS penetration
SOLUBILITYWater soluble; nasal spray formulation available
CAS NO.80714-61-0
STATUSFDA Approved
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TAGS
cognitive enhancementneuroprotectionBDNFstrokeADHDanxiety