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ANTIMICROBIALPreclinicalANTIMICROBIAL PEPTIDEANTICANCERFROG-DERIVED

Brevinin-1 E8.13

Also known as: Brevinin-1E8.13

1 views/week 0 citations 0 edits Updated 8/30/2026

Brevinin-1 E8.13 is a 24-residue antimicrobial and anticancer peptide cloned from the skin secretion of Sylvirana guentheri, a frog native to Ha Giang, Vietnam, published in Current Issues in Molecular Biology (August 2025). Preclinical, in-vitro research only — no human trials.

STRUCTURE

Molecular Composition

FORMULA
C₁₁₉H₁₉₄N₂₈O₂₈S₂ (calc. from sequence)
MOL. WEIGHT
2,529.14 Da
SEQUENCE LENGTH
24 amino acids
ORIGIN
Sylvirana guentheri (frog skin)
NET CHARGE
+4
STATUS
Preclinical only
AMINO ACID CHAIN VISUALIZATION
F
Phenylalanine
N-terminal hydrophobic anchor
NH-CO
L
Leucine
membrane insertion
NH-CO
K
Lysine
cationic charge, bacterial membrane binding
NH-CO
K
Lysine
cationic charge, bacterial membrane binding
NH-CO
C
Cysteine
disulfide "Rana box" ring formation
NH-CO
C
Cysteine
disulfide "Rana box" ring formation
SEQUENCEF-L-K-K-C-C
MECHANISMS

How It Works

🦠
Membrane-Disrupting Antimicrobial Action
A net +4 charge and ~62% hydrophobic content drive electrostatic binding to negatively charged bacterial membranes, causing destabilization and lysis — the classic cationic-antimicrobial-peptide mechanism shared with other Brevinin-1-family peptides.
🎯
Selective Cancer Cell Cytotoxicity
Above a concentration threshold (roughly 7.5–15.8 μM depending on cell line), the peptide forms pores in cancer cell membranes causing ion leakage and rapid cell death, while showing low toxicity — and even promoting proliferation at lower concentrations — in normal dermal fibroblasts.
🔬
Cyp1a1 Downregulation in Liver Cancer Cells
Beyond direct membrane disruption, the peptide was shown to downregulate Cyp1a1 gene expression in liver cancer cells, a secondary mechanism that may interfere with carcinogenic metabolic pathways.
OVERVIEW

Research Overview

Brevinin-1 E8.13 showed potent activity against Staphylococcus aureus (MIC 1.5 μM) and cytotoxicity against several human cancer cell lines (A549, AGS, Jurkat, HCT116, HL60, HepG2; IC50 7.5–31.6 μM), while sparing and even promoting proliferation of normal human dermal fibroblasts at lower concentrations.

This is a single published preclinical study — no human trials, no established dosing, and no confirmed clinical development pathway at time of writing.

Mechanism of Action

A net +4 charge and ~62% hydrophobic content drive electrostatic binding to negatively charged bacterial membranes, causing destabilization and lysis — the classic cationic-antimicrobial-peptide mechanism. Above a concentration threshold, the peptide forms pores in cancer cell membranes causing ion leakage and rapid death, and was also shown to downregulate Cyp1a1 gene expression in liver cancer cells.

DOSAGE

Dosage & Administration

Brevinin-1 E8.13 is a 24-residue antimicrobial/anticancer peptide (sequence FLGALFKVASKLVPAAICSFSKKC) cloned from the skin secretion of Sylvirana guentheri, a frog native to Ha Giang, Vietnam, and published in Current Issues in Molecular Biology (August 2025). This is preclinical, in-vitro research only — no human trials, no established dosing, and no clinical development pathway confirmed at time of writing. Showed potent activity against Staphylococcus aureus (MIC 1.5 μM) and cytotoxicity against several human cancer cell lines (IC50 7.5–31.6 μM) while sparing normal dermal fibroblasts. There is no established human dose, and research-grade sourcing and purity are entirely unregulated.

CYCLING

Cycle Duration Guide

ON CYCLE
Not applicable — preclinical research compound, no human dosing established
OFF CYCLE
Not applicable

No cycling protocol exists because this peptide has not been studied in humans.

Preclinical / in-vitro only — a single published study, no human data. Not a research compound with an established use pattern.

NOTES

Research Notes

Preclinical / in-vitro only. No human dosing established. Research-grade sourcing and purity for this compound are entirely unregulated, and no clinical development pathway has been confirmed.
Quick Reference
FORMULAC₁₁₉H₁₉₄N₂₈O₂₈S₂
MOL. WEIGHT2,529.14 Da
LENGTH24 amino acids
ORIGINSylvirana guentheri (frog) skin secretion; recombinant/synthetic for study
HALF-LIFENot established (preclinical only)
SOLUBILITYNot established (preclinical only)
STATUSPreclinical
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TAGS
antimicrobial peptideanticancerfrog-derivedpreclinicalbrevinin family